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The word best is an adjective, and adjectives do not take articles by themselves. This is very good instinct, and you could. In the following sentence, however, best is an adjective:

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Which one is the best is obviously a question format, so it makes sense that which one the best is should be the correct form.

Your example already shows how to use best as an adverb.

Best wishes 和 best regards 有什么区别?首先我们来看下best wishes和best regards的大致意思：best wishes：词性为短语，用于表示对某人的最好祝愿，通常用于书信、贺卡等正式场合. What was best? if we insert the word the, we get a noun phrase, the best. It is also a superlative, like greatest, or highest, so just as you would use it as an adjective to show that something is.